Date of Award
1992
Document Type
Senior Scholars Paper (Open Access)
Department
Colby College. Chemistry Dept.
Advisor(s)
L. Daniel Libby
Second Advisor
Wayne Smith
Third Advisor
Peter Harris
Abstract
The mechanism of chloroperoxidase (CPO)-catalyzed peroxidatic reactions of several substituted hydroquinones was studied at various hydrogen peroxide concentrations. The pathway was studied using cytochrome c as the radical trapping agent. As the hydroquinones became more hindered there was a difference in the amount of radicals trapped. For hydroquinone, 59.3% radical pathway, and methylhydroquinone, 81.4% radical, the difference in radicals trapped is due to a difference in pathway. For 2,3-dimethylhydroquinone (75.4%), trimethylhydroquinone (44.5%), and t-butylhydroquinone (0%) other non-peroxidatic reactions are noticed. Thus, for the more substituted hydroquinones the difference in radicals trapped can not be assigned to a difference in radical pathway. Also, there were problems drawing conclusions for this system due to the catalytic reaction of hydrogen peroxide. The radical trapping ability of 2,4,6-trimethylphenol was investigated for various other substrates. TMP reacted with the radicals generated in the enzymatic reactions of phenol, resorcinol, and m-methoxyphenol. Thus, this TMP system offers further potential as another radical trapping agent for use in these studies.
Keywords
Free radicals (Chemistry)
Recommended Citation
Provencal, David P., "Involvement of free radicals in peroxidatic reactions catalyzed by chloroperoxidase" (1992). Senior Scholar Papers. Paper 220.https://digitalcommons.colby.edu/seniorscholars/220
Copyright
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