Date of Award
Honors Thesis (Open Access)
Colby College. Chemistry Dept.
Kevin P. Rice
Laromustine is a sulfonylhydrazine anticancer prodrug whose main cytotoxic arises from its ability to interfere with DNA replication of dividing cells. Multiple studies have suggested that Laromustine induces a form of cell death known as apoptosis. In a previous study investigating the mechanism of apoptosis, bcl2-associated athanogene 3 (BAG3) demonstrated significant upregulation in the presence of Laromustine. Given its anticancer ability, we selected BAG3 as a target for further investigation. BAG3 knockdown through transient shRNA transfections was performed in U138 glioblastoma multiforme cells and verified using qRT-PCR analysis. Finally, cell death assays were used to assess Laromustine’s cytotoxic effect on BAG3 knockdown cells.
BAG3, Laromustine, Leukemia, Apoptosis
Recommended CitationSheehan, Colin, "An Investigation of BAG3 Knockdown and its Effects on the Cytotoxicity of Laromustine" (2015). Honors Theses. Paper 760.
Colby College theses are protected by copyright. They may be viewed or downloaded from this site for the purposes of research and scholarship. Reproduction or distribution for commercial purposes is prohibited without written permission of the author.