Date of Award
2015
Document Type
Honors Thesis (Open Access)
Department
Colby College. Chemistry Dept.
Advisor(s)
Kevin P. Rice
Second Advisor
Nicholas Boekelheide
Abstract
Laromustine is a sulfonylhydrazine anticancer prodrug whose main cytotoxic arises from its ability to interfere with DNA replication of dividing cells. Multiple studies have suggested that Laromustine induces a form of cell death known as apoptosis. In a previous study investigating the mechanism of apoptosis, bcl2-associated athanogene 3 (BAG3) demonstrated significant upregulation in the presence of Laromustine. Given its anticancer ability, we selected BAG3 as a target for further investigation. BAG3 knockdown through transient shRNA transfections was performed in U138 glioblastoma multiforme cells and verified using qRT-PCR analysis. Finally, cell death assays were used to assess Laromustine’s cytotoxic effect on BAG3 knockdown cells.
Keywords
BAG3, Laromustine, Leukemia, Apoptosis
Recommended Citation
Sheehan, Colin, "An Investigation of BAG3 Knockdown and its Effects on the Cytotoxicity of Laromustine" (2015). Honors Theses. Paper 760.https://digitalcommons.colby.edu/honorstheses/760
Copyright
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