Date of Award
Honors Thesis (Open Access)
Colby College. Biology Dept.
Andrea R. Tilden
Julie T. Millard
Melatonin (N-acetyl-5-methoxytryptamine) is an indolamine hormone produced by the pineal gland that works to regulate sleep/wake cycles and activity rhythms. The effects of melatonin in metabolism are far from understood. Melatonin was injected into the fiddler crab, Uca pugilator, to investigate the effects of melatonin on hemolymph glucose and lactate levels. Following injection at t=O, hemolymph samples were collected at t=O.5, 1.0, 1.5 and 5.0 hours. Melatonin caused a decrease in the stress response to injection and also caused delayed hyperglycemia. Melatonin-injected crabs also retained the glucose and lactate rhythymicity when compared to saline-injected crabs. Glucose and lactate rhythms followed the same pattern indicating that the cycles are coupled. Also, melatonin was synthesized using tbe Fischer Indole synthesis and characterized using H?NMR. The synthetic melatonin demonstrated biological activity when injected into the crabs as when compared to pure melatonin on the effects on glucose and lactate concentrations. Overall, melatonin influences both glucose metabolism and the production of lactate.
Melatonin -- Physiological effect, Blood sugar, Lactates, Fiddler crabs
Recommended CitationDalton, Kathryn M., "Effects of melatonin on hemolymph glucose and lactate concentrations in the fiddler crab, uca pugilator" (2002). Honors Theses. Paper 66.
Colby College theses are protected by copyright. They may be viewed or downloaded from this site for the purposes of research and scholarship. Reproduction or distribution for commercial purposes is prohibited without written permission of the author.
Click below to download supplemental content.Dalton 1.jpg (87 kB)
Dalton 2.jpg (140 kB)
Dalton 3.jpg (187 kB)
Dalton 4.jpg (305 kB)
Dalton 5.jpg (306 kB)