Date of Award
Honors Thesis (Open Access)
Colby College. Chemistry Dept.
D. Whitney King
A flow injection system using the chemiluminescent reagent 2-methyl-6-(4methoxyphenyl)- 3,7-dihydroimidazo[1,2-a]pyrazin-3-one (MCLA) was used to quantify aqueous concentrations of superoxide. Stock solutions of superoxide were produced by photolysis of a solution of 2-propanol and benzophenone. The concentration of superoxide was quantified in the stock solutions by direct measurement of the absorbance at 240 nm. Quantified additions of superoxide to the flow injection analysis system reacted with MCLA at pH 5.1 demonstrated a detection limit of 0.074 nM superoxide. Selective superoxide detection was confirmed by pH-dependent decay of superoxide, which was in excellent agreement with published rates.
Superoxide, Solution (Chemistry), Chemiluminescence
Recommended CitationBoland, Nathan, "Quantification of nanomolar superoxide in aqueous solution : flow injection analysis using the chemiluminescent reagent MCLA." (2001). Honors Theses. Paper 36.
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