Date of Award
Honors Thesis (Open Access)
Colby College. Chemistry Dept.
Shari U. Dunham
In order to understand further the interactions that exist between specific organometallic complexes and DNA, reactions of double-stranded calf-thymus DNA with bis(cyclopentadienyl)titanium dichloride [Cp₂TiC1₂] (TDC), bis(t-butylcyclopentadienyl)titanium dichloride [(C₄H₉Cp)₂TiC1₂] (r-butyl TDC), bis(i-propylcyclopentadienly)titanium dichloride [(C₃H₆Cp)2TiCl₂ a-propyl mc), bis(pentamethylcyclopentadienyl) titanium dichloride [(Cp"₂TiC1₂ (pentarnethyl TDC), triphenyltin chloride (Ph₃SnCl), diphenylrin dichloride (Ph₂SnC1₂) , and triphenyltin acetate (Ph₃SnOAc) were performed. The goal of this research is to establish how the fundamental structure and composition of these compounds determine the effecti veness of their DNA interactions. Each metal compound was incubated with calf thymus DNA at several metal: DNA ratios and reactions were maintained at 37°C at a pH of 6 or 8. Multiple time points were taken of each reaction mixture and for each time point the modified DNA was isolated via ethanol precipitation. Modified DNA was then analyzed by ICP-AES (to determine the amount of bound metal), by Picotrreen" fluorescence spectroscopy (to determine the amount of DNA) and by HPLC separation of enzymatic digestions (to determine the relative percent area of each of the four bases in modified and control DNA). The titanium compounds were also incubated with a synthetic 11 base pair oligomer of double stranded DNA at room temperature and analyzed with HPLC and ICP to determine the amount of bound Ti to the DNA over time. Preliminary data is presented and DNA-binding trends will be discussed.
Organometallic compounds, DNA Organotin compounds, organometallic complexes, DNA interaction
Recommended CitationDavies, Katherine MacDonald, "Interactions of Ti(IV) and Sn(IV) Organometallic Complexes with Double-stranded DNA" (2000). Honors Theses. Paper 254.
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