Author (Your Name)

Jason D. Hosmer, Colby College

Date of Award


Document Type

Honors Thesis (Open Access)


Colby College. Chemistry Dept.


D. Whitney King


A means of determining hydroxyl radical production rates in natural waters through the formation of a directly detectable fluorescent product has been developed. A method similar to the enzyme mediated oxidation of phydroxyphenylacetic acid by hydrogen peroxide is used (Miller and Kester, 1988). Due to the high reactivity of hydroxyl radicals, and subsequently low steady state concentrations, direct detection is very difficult. In the past detection was achieved through the introduction of a probe, usually a substituted aromatic or alcohol, that decays away at an observable rate upon reaction with hydroxyl radicals. Detection limits and accuracy of such difference based techniques are typically poor, and do not provide an adequate analytical technique.


Water chemistry, Hydroxyl group, hydroxyl radical production, rate, fluorometric reagent p-hydroxyphenylacetic acid, natural waters

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