Event Title
The Effect of Phosphorylation on Transcription Factor TaABF1’s Ability to Downregulate the Amy32b gene in Cereal Aleurone
Location
Diamond 153
Start Date
1-5-2014 1:00 PM
End Date
1-5-2014 2:50 PM
Project Type
Presentation
Description
The transcription factor TaABF1 plays an important role in mediating hormone-induced changes in gene expression in cereal grains, allowing for seeds to respond properly to varying environmental conditions. It is necessary for the abscisic acid-induced suppression of Amy32b, a gene encoding an α-amylase in cereal grains, such as wheat and barley. In order to examine the relevance of the phosphorylation state of TaABF1 in its ability to downregulate Amy32b, we generated mutant TaABF1 constructs with aspartate or alanine substituted for serines in conserved regions in the protein to mimic the presence or absence of phosphate. Through particle bombardment, it was determined that phosphorylation at conserved region one enhances TaABF1s ability to inhibit expression of Amy32b compared to the unmodified wild-type transcription factor.
Faculty Sponsor
Cathy Bevier
Sponsoring Department
Colby College. Biology Dept.
CLAS Field of Study
Natural Sciences
Event Website
http://www.colby.edu/clas
ID
443
The Effect of Phosphorylation on Transcription Factor TaABF1’s Ability to Downregulate the Amy32b gene in Cereal Aleurone
Diamond 153
The transcription factor TaABF1 plays an important role in mediating hormone-induced changes in gene expression in cereal grains, allowing for seeds to respond properly to varying environmental conditions. It is necessary for the abscisic acid-induced suppression of Amy32b, a gene encoding an α-amylase in cereal grains, such as wheat and barley. In order to examine the relevance of the phosphorylation state of TaABF1 in its ability to downregulate Amy32b, we generated mutant TaABF1 constructs with aspartate or alanine substituted for serines in conserved regions in the protein to mimic the presence or absence of phosphate. Through particle bombardment, it was determined that phosphorylation at conserved region one enhances TaABF1s ability to inhibit expression of Amy32b compared to the unmodified wild-type transcription factor.
https://digitalcommons.colby.edu/clas/2014/program/27