Author (Your Name)

Donna L. Webber

Date of Award


Document Type

Senior Scholars Paper (Open Access)


Colby College. Chemistry Dept.


George D. Maier

Second Advisor

Evans B. Reid

Third Advisor

Wayne Smith


Crude renin preparations from steer kidneys were incubated with Cd2+ and with Zn2+ elevation of renin activity resulted in both preparations. Analyses of each preparation against Tris-saline, and finally EDTA, removed the cations; however, renin activity remained elevated. An indirect activation of Renin by Cd2+ and, to a lesser extent, Zn2+, was thus demonstrated. Removal of the cations by Tris-saline and DTA-dialyses was suspected to involve the loss by dialysis of a renin inhibitor. An isolation technique for a proposed phospholipid renin inhibitor similar to bovine phosphatidylserine, reported by another laboratory, was thought to be unnecessarily arduous due to the probable location of an inhibitor in the Tris-saline and EDTA-dialysands (the sample outside the dialysis sacs) of Cd2+ and Zn2+-incubated renin samples. Extractions of Tris-saline-and EDTA-dialysands of Cd 2+-and Zn2+-incubated renin samples for a phospholipid renin inhibitor were attempted using various mixtures of organic and polar solvents. Renin samples incubated with reconstituted "inhibitor" extractions did not demonstrate marked or total activity depression.


kidney disease, hypertension, renin, renal hypertension, renin inhibitor, dializable phospholipid

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