Date of Award
1970
Document Type
Senior Scholars Paper (Open Access)
Department
Colby College. Chemistry Dept.
Advisor(s)
George D. Maier
Second Advisor
Evans B. Reid
Third Advisor
Wayne Smith
Abstract
Crude renin preparations from steer kidneys were incubated with Cd2+ and with Zn2+ elevation of renin activity resulted in both preparations. Analyses of each preparation against Tris-saline, and finally EDTA, removed the cations; however, renin activity remained elevated. An indirect activation of Renin by Cd2+ and, to a lesser extent, Zn2+, was thus demonstrated. Removal of the cations by Tris-saline and DTA-dialyses was suspected to involve the loss by dialysis of a renin inhibitor. An isolation technique for a proposed phospholipid renin inhibitor similar to bovine phosphatidylserine, reported by another laboratory, was thought to be unnecessarily arduous due to the probable location of an inhibitor in the Tris-saline and EDTA-dialysands (the sample outside the dialysis sacs) of Cd2+ and Zn2+-incubated renin samples. Extractions of Tris-saline-and EDTA-dialysands of Cd 2+-and Zn2+-incubated renin samples for a phospholipid renin inhibitor were attempted using various mixtures of organic and polar solvents. Renin samples incubated with reconstituted "inhibitor" extractions did not demonstrate marked or total activity depression.
Keywords
kidney disease, hypertension, renin, renal hypertension, renin inhibitor, dializable phospholipid
Recommended Citation
Webber, Donna L., "A Dialyzable Phospholipid Renin Inhibitor" (1970). Senior Scholar Papers. Paper 369.https://digitalcommons.colby.edu/seniorscholars/369
Copyright
Colby College theses are protected by copyright. They may be viewed or downloaded from this site for the purposes of research and scholarship. Reproduction or distribution for commercial purposes is prohibited without written permission of the author.