Presenter Information

Tarini Hardikar, Colby CollegeFollow

Location

Parker-Reed, SSWAC

Start Date

1-5-2014 9:00 AM

End Date

1-5-2014 10:00 AM

Project Type

Poster

Description

The halophilic archaeon Halobacterium salinarum survives in low-oxygen conditions by producing a light-driven proton pump, bacteriorhodopsin (BR). BR is formed by a retinal cofactor covalently bound to the protein bacterioopsin (BO). In reduced oxygen conditions, H. salinarum synthesizes BR, which requires production of both BO and the retinal cofactor. The gene that encodes for BO is called bacterio-opsin protein gene (bop). In several organisms, it has been observed that another gene, bacterio-opsin associated protein gene (bap) is located next to the bop gene. However, the function of bap is unknown. To identify whether the bap gene has any role in BO or BR production, we deleted the bap gene in H. salinarum.

Sponsoring Department

Colby College. Biology Dept.

CLAS Field of Study

Natural Sciences

Event Website

http://www.colby.edu/clas

ID

346

Included in

Biology Commons

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May 1st, 9:00 AM May 1st, 10:00 AM

Identifying the Role of Bacterio-opsin Associated Protein Gene in Bacteriorhodopsin Production in the Halophilic Archaeon Halobacterium salinarum

Parker-Reed, SSWAC

The halophilic archaeon Halobacterium salinarum survives in low-oxygen conditions by producing a light-driven proton pump, bacteriorhodopsin (BR). BR is formed by a retinal cofactor covalently bound to the protein bacterioopsin (BO). In reduced oxygen conditions, H. salinarum synthesizes BR, which requires production of both BO and the retinal cofactor. The gene that encodes for BO is called bacterio-opsin protein gene (bop). In several organisms, it has been observed that another gene, bacterio-opsin associated protein gene (bap) is located next to the bop gene. However, the function of bap is unknown. To identify whether the bap gene has any role in BO or BR production, we deleted the bap gene in H. salinarum.

http://digitalcommons.colby.edu/clas/2014/program/403