Event Title

Spatial and Temporal Distribution of Pseudo-nitzschia in the Gulf of Maine

Presenter Information

Sonia Vargas, Colby CollegeFollow

Location

Parker-Reed, SSWAC

Start Date

1-5-2014 9:00 AM

End Date

1-5-2014 10:00 AM

Project Type

Poster- Restricted to Campus Access

Description

Pseudo-nitzschia (PN) is biologically interesting because it is the only marine diatom known to produce harmful algal blooms (HABs). Due to the potential human and marine mammal health risks of a large bloom of PN, researchers, fisheries, public health officials, and conservationists worldwide are interested in monitoring the distribution and abundance of this diatom in the ocean. By using molecular methods and the analysis of the 18S rRNA gene of the genus Pseudo-nitzschia (PN), we hope to obtain a greater understanding of the distribution of PN populations in the Gulf of Maine. A quantitative PCR (qPCR) assay based on the use of SYBR Green I detection was performed to assess the abundance of the genus Pseudo-nitzschia in whole water samples over time and location in the Damariscotta River Estuary and the Gulf of Maine. We found that over our time-series transect, a relatively large bloom of PN occurred offshore in early October, and sunk to depth by mid-November. These bloom dynamics may have implications for future PN populations and populations closer to shore.

Sponsoring Department

Colby College. Biology Dept.

CLAS Field of Study

Natural Sciences

Event Website

http://www.colby.edu/clas

ID

257

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May 1st, 9:00 AM May 1st, 10:00 AM

Spatial and Temporal Distribution of Pseudo-nitzschia in the Gulf of Maine

Parker-Reed, SSWAC

Pseudo-nitzschia (PN) is biologically interesting because it is the only marine diatom known to produce harmful algal blooms (HABs). Due to the potential human and marine mammal health risks of a large bloom of PN, researchers, fisheries, public health officials, and conservationists worldwide are interested in monitoring the distribution and abundance of this diatom in the ocean. By using molecular methods and the analysis of the 18S rRNA gene of the genus Pseudo-nitzschia (PN), we hope to obtain a greater understanding of the distribution of PN populations in the Gulf of Maine. A quantitative PCR (qPCR) assay based on the use of SYBR Green I detection was performed to assess the abundance of the genus Pseudo-nitzschia in whole water samples over time and location in the Damariscotta River Estuary and the Gulf of Maine. We found that over our time-series transect, a relatively large bloom of PN occurred offshore in early October, and sunk to depth by mid-November. These bloom dynamics may have implications for future PN populations and populations closer to shore.

http://digitalcommons.colby.edu/clas/2014/program/380