Presenter Information

Rachel Bolender, Colby CollegeFollow

Location

Parker-Reed, SSWAC

Start Date

1-5-2014 9:00 AM

End Date

1-5-2014 10:00 AM

Project Type

Poster

Description

Magnetotactic bacteria (MTB) are a group of bacteria that synthesize magnetosomes, magnetic crystals of magnetite (Fe304) or greigite (FeS4), which allow MTB to travel along the earths geomagnetic fields. In addition, MTB have been shown to be promising agents for bioremediation through their ability to absorb heavy metals, such as gold. Little is known of the genetic elements that allow for the biosynthesis of magnetic particles in MTB. In this study, genes MamB and MamM, genes known to be involved in magnetosome formation but whose functions are not known, were studied using the magnetotactic bacteria Magnetospirillum magneticum. Genomic DNA was extracted from M. magneticum, primers for MamB and MamM were designed, and these genes were amplified via PCR. PCR products were inserted into expression vectors and used to transform E. coli. The ability of E. coli transformed with MamB and MamM to absorb gold from solution is examined in this study.

Faculty Sponsor

Ron Peck

Sponsoring Department

Colby College. Biology Dept.

CLAS Field of Study

Natural Sciences

Event Website

http://www.colby.edu/clas

ID

785

Included in

Biology Commons

Share

COinS
 
May 1st, 9:00 AM May 1st, 10:00 AM

MamB and MamM: Genes Involved in Magnetotaxis

Parker-Reed, SSWAC

Magnetotactic bacteria (MTB) are a group of bacteria that synthesize magnetosomes, magnetic crystals of magnetite (Fe304) or greigite (FeS4), which allow MTB to travel along the earths geomagnetic fields. In addition, MTB have been shown to be promising agents for bioremediation through their ability to absorb heavy metals, such as gold. Little is known of the genetic elements that allow for the biosynthesis of magnetic particles in MTB. In this study, genes MamB and MamM, genes known to be involved in magnetosome formation but whose functions are not known, were studied using the magnetotactic bacteria Magnetospirillum magneticum. Genomic DNA was extracted from M. magneticum, primers for MamB and MamM were designed, and these genes were amplified via PCR. PCR products were inserted into expression vectors and used to transform E. coli. The ability of E. coli transformed with MamB and MamM to absorb gold from solution is examined in this study.

http://digitalcommons.colby.edu/clas/2014/program/347